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Leica LASX software was used to create a maximum projection image to be applied for subsequent analyses in ImageJ. The XY location of CD8 + objects and CXCL13 + objects, and fluorescence colocalization of CXCR5 staining in CD8 + objects were determined in ImageJ.
Nov 21, 2017 · Then the scaffolds were imaged using a Leica DM5500 upright microscope (Leica Microsystems, Buffalo Groove, IL) and analyzed with Leica LASX software. MTT assay. MC3T3 cells were seeded onto 1 cm × 1 cm × 0.5 cm foams of 0%, 2.5%, 5%, and 10% gelatin concentrations. Cells were incubated for 60 h at 37 °C.
Feb 23, 2018 · Imaging was done using Leica SP5 confocal microscope equipped with LasX software. Transgenic mice and ganciclovir regimen Selective ablation of SC-NSCs was done in Nestin TK mice that were originated from Nestin flox GFP flox -TK mice [ 19 ] in which GFP excision was done using CMV-Cre mouse line [ 23 ].
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Apr 13, 2019 · Leica Application Suite X (LAS X, LASX) is a platform for advanced life science research on Leica Microsystems confocal, widefield and super-resolution systems. Obtain reproducible analysis results rapidly and easily with the 3D and 2D image analysis wizards.
After washing for 8 h at room temperature, sections were mounted in Mowiol for confocal imaging using a Leica SP8 TCS confocal microscope using the DAPI diode laser (405 nm) and white light laser. Lasers were set to 405 nm at 20% power, 488 nm at 7% power, 550 nm at 10% power and 594 nm at 4% power.
Confocal images of chlamydial inclusions were captured on a Leica TCS SP8 with Leica LASX software at 630x or 1,000x magnification. Imaris imaging and Leica LASX software were used to analyze the images. All images used in intensity measurements were taken at a standard exposure.
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  • LAS X, Free Download by Leica Microsystems CMS GmbH. Casino Las Vegas - Superb 3D Games. 400$ Cash Bonus! NEW VERSION 2008V.
  • In July 2018, reports have reached us that the HyVolution 2 module in the LAS X software provides partly incorrect multi-channel meta-data to Huygens. This is known to occur when multi-channel LIF files are read into LASX and subsequently sent to Huygens via Hyvolution2. Consequently, this may result in non-optimal deconvolution results.
  • LASX Software equipped with Navigator; Focus Mapping and Lightning( A patented Deconvolution software) (NEW) Other Imaging techniques: FRAP, FRET, Photostimulation, and Spectral Imaging Excellent for Live imaging, Subcellular Protein or gene Localisation and super-resolution imaging

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Nov 13, 2019 · Hippocampal dentate gyrus imaging was performed using confocal microscope (Leica STED). Whole area of dentate gyrus was visualized by mosaic stitch procedure of Leica LasX. DCX + cells were counted in whole dentate gyrus images of hippocampus. The obtained cell numbers were normalized by the size of dentate gyrus (DCX + cell number/mm 2).

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Apr 23, 2021 · Leica LAS AF Lite is a platform designed for advanced life science research with Leica Microsystems confocal and widefield systems. The program offers full control over the microscope hardware and at the same time minimizes the need for user interaction through intelligent automation.

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Software Leica LasX High Resolution 10-Megapixel Camera 2D and 3D Measurements XY-Stage, Manual and Motorized Focus Drive (coarse/fine), Manual and Motorized Tilting Angle, Continuous Adjustment from ± 60° Illumination: Transmitted and Reflective

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Les logiciels Windows les plus populaires et les plus téléchargés, y compris les meilleurs Antivirus Gratuits de 2016, les outils d'Optimisation Système et les Lecteurs Multimédia.


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Jul 21, 2020 · INTRODUCTION. Although artists’ illustrations of the prototypical diploid mammalian cells show a centrally located, radially symmetric nucleus, the position of the cell nucleus is known to vary based on developmental timing, differentiation state, motile behavior, and other physiological factors (Gundersen and Worman, 2013).

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Apr 04, 2016 · Leica Sport Optics now have a 10-year Passport protection plan and a 30-year warranty; The Leica Q2 Daniel Craig x Greg Williams limited edition camera is already sold out; Leica will launch more new M lenses with a minimum focusing distance of 30cm; Leica to announce a new VARIO-ELMARIT-SL 24–70mm f/2.8 ASPH lens

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Three-dimensional (3D) reconstructions of pictures were created in Leica LasX software. The minimal size of 10 μm 2 was selected for identifying normal SPG nuclei. For PG and CG, a minimal size of 3 μm 2 was selected. Figures and illustrations were prepared in Leica LasX and Adobe Photoshop CC 2019 (Adobe, USA) (Supplementary Figure S1).

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Single z planes were extracted in tiff format with Fiji software (v 1.0) (Schindelin et al, 2012). The brightness and contrast of the images (from wide‐field and confocal microscopy) in the figures were adjusted using Photoshop CS5 (Adobe Version 12.0.4x64; RRID:SCR_014199 ).

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Images were taken with a Leica DM 5500B Imaging system (Leica Microsystems, Bensheim, Germany) with a Leica DC500 camera, using Leica application suite (LASX) software. Immunohistochemistry (IHC) To address ECM alterations, the following immunohistochemical stains were performed.

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Single z planes were extracted in tiff format with Fiji software (v 1.0) (Schindelin et al, 2012). The brightness and contrast of the images (from wide‐field and confocal microscopy) in the figures were adjusted using Photoshop CS5 (Adobe Version 12.0.4x64; RRID:SCR_014199 ).

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Jul 21, 2020 · INTRODUCTION. Although artists’ illustrations of the prototypical diploid mammalian cells show a centrally located, radially symmetric nucleus, the position of the cell nucleus is known to vary based on developmental timing, differentiation state, motile behavior, and other physiological factors (Gundersen and Worman, 2013).

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3 Leica MZ16 FA – User manual Operating controls Additional operating controls, see page 38. Fig. 1 Leica MZ16 FA with ErgoTube® 1 Display with function keys 2 Motorized filter changer with four filter holders 3 Filter holder for an individually selectable filter 4 ErgoTube® with variable viewing angle of 10° – 50 5 Adjustable eyepiece ...

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Jan 15, 2020 · Images were acquired using a Leica DM5000 light microscope with 40× 0.85NA HC Plan Apo and 100× 1.4NA HCX Plan Apo objectives. DFC 550 top-mount camera and Leica LASX software were used. IV and ICV treatment in cynomolgus monkey

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Leica Application Suite X (LAS X , LASX) is a platform for advanced life science research on Leica Microsystems confocal, widefield and super-resolution systems.Obtain reproducible analysis results rapidly and easily with the 3D and 2D image analysis wizards.

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Confocal microscopy was performed on a Leica SP8 inverted confocal system (Leica Microsystems) equipped with a HC PL APO 63×/1.40 numerical aperture oil immersion lens. Scanning was performed in line-by-line sequential mode. Images were captured and processed using LasX (Leica Microsystems) software.

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applied in the coverslip mounting medium. Slides were imaged by confocal imaging Leica LasX software system (Leica, Buffalo Grove, IL, USA). Microglia size measurements were completed by Leica Application Suite analysis software (Leica, Buffalo Grove, IL, USA) and Image J. Images were taken at 40x with a

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The recorded image stacks were deconvolved using Leica Lightning processing in LASX. Automated calculation of perisomatic bouton density was performed offline in MatLab (MathWorks) using the Image Processing Toolbox and custom-written routines.

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Jun 30, 2020 · Expression levels of 1-deoxysphingosine staining were semiquantitatively analyzed using LASX software (Leica, Wetzlar, Germany). Results are expressed as mean±standard deviation of optical density of six different digital images. ** p<0.01 and *** p<0.001. X-ray before-PL, the group of that PL was treated before X-ray radiation, UVB before-PL ...

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The present invention generally relates to various dibenzothiophene compounds, processes for preparing these compounds, and use of these compounds in organic light-emitting diodes and as cellular imag

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This video demonstrates one method of importing data into Leica Icon software. This involves first creating a project in Icon Construct, and ensuring the des...

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This video provides a brief overview of the user interface or UI in Leica Application Suite EZ more commonly referred to as LAS EZ.

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History. Between 1960 and 1973, Leitz (Leica) patented an array of autofocus and corresponding sensor technologies. At photokina 1976, Leica had presented a camera based on their previous development, named Correfot, and in 1978 they displayed an SLR camera with fully operational autofocus.

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Leica Application Suite X (LAS X) from Leica Microsystems is the software platform for straightforward imaging and documentation in industrial applications. Leica LAS X helps you to deliver reliable results with confidence. Speed, efficiency, and precision drive your team, powered by this software that facilitates their daily tasks

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How to get vaniqa online. This basic-level eLearning course provides the most common cancer among women who have different political party affiliations or are ...

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The Leica HyD can be integrated into any new or existing Leica TCS SP8 system. With its high quantum efficiency, low noise and large dynamic range, the Leica HyD is the most versatile detector in the Leica TCS SP8 confocal platform. It synergizes perfectly with our filter-free spectral detection system and the acousto-optical beam splitter ...

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Hyperosmolarity and dryness conditions have determined THP-1 cells migration and infiltration: CD68+ was relevant for macrophages, CD83+ for dendritic cells. CD14 was a marker of undifferentiated THP-1 cells. Quantification of the number of migrated cells have been performed by using the software Leica LASX applied to LEICA DM2500.

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Nov 21, 2017 · Then the scaffolds were imaged using a Leica DM5500 upright microscope (Leica Microsystems, Buffalo Groove, IL) and analyzed with Leica LASX software. MTT assay. MC3T3 cells were seeded onto 1 cm × 1 cm × 0.5 cm foams of 0%, 2.5%, 5%, and 10% gelatin concentrations. Cells were incubated for 60 h at 37 °C.

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Biopsies were sliced longitudinally through the injection site and mounted on a microscopy slide with a 1 mm depression in RPMI 10% FCS. Slides were imaged for 30-60 consecutive minutes using a Leica TSC SP8 Confocal microscope with accompanying Leica LASX software, using an exposure time of approximately 1.7 seconds per frame and a 40x objective.

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Live cell imaging was done at 30-second time intervals for a total duration of 4.5 hours using a Leica DFC9000 GT camera (Leica Mikroskopie & Systeme Ltd., Wetzlar, Germany), a CoolLEDpE4000 light source (CoolLED Ltd., Andover, Great Britain), and Leica Application Suite X Software (LAS X 3,0,4,16529, Leica Mikroskopie & Systeme Ltd., Wetzlar, Germany).

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Leica ipc software Free Download for Windows. ... Leica Application Suite X (LAS X, LASX) is a platform for advanced life science research on Leica Microsystems ...

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Life Science Software Informer. Featured Life Science free downloads and reviews. Latest updates on everything Life Science Software related.

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www.impactournals.comoncotarget 91915 Oncotarget comes from studies with 2,3,7,8-tetrachlorodibenzodiox in (TCDD). First described as the TCDD receptor, ligand-

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徕卡显微系统(Leica Microsystems)是具有160余年悠久历史的德国著名的光学制造企业,也是目前同行业中唯一能够同时提供显微镜、图像采集产品、图像分析软件的厂商。徕卡显微系统是全球创新显微镜学,显微摄像,及软件解决方案的领导者,提供宏观,微观及纳米结构的影像及分析服务。
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For more information on the software features, please view Image Analysis Software.. Researchers who are interested in our Image Work Processing Station, please contact Yang Fan


TMRM was excited using the 552 nm laser, and fluorescence collected using a 560–630 nm band pass filter. MitoTracker Green was excited using the 488 nm laser and fluorescence collected using a 495–540 nm band pass filter. Confocal sections obtained from the LASX software (Leica) were analyzed using ImageJ for measurements of MMP.